Low Temperature Physics: 48, 318 (2022); https://doi.org/10.1063/10.0009736
Fizika Nizkikh Temperatur: Volume 48, Number 4 (April 2022), p. 359-363    ( to contents , go back )

The spectral investigations of interaction between high-molecular proteins and small adenine derivates

V. Yu. Kudrya1, V. M. Yashchuk1, Z. Yu. Tkachuk2, Ya. O. Gumenyuk3, and A. P. Naumenko 1

1Physics Faculty, Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine
E-mail: vladkudrya@ukr.net, a_naumenko@univ.kiev.ua

2Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv 03143, Ukraine

3National University of Life and Environmental Sciences of Ukraine, Kyiv 03041, Ukraine
pos Анотація:756

Received November 30, 2021, published online February 25, 2022

Abstract

The spectral properties of co-systems of the DNA, RNA, and small nucleotide derivates with high-molecular π-electron-containing proteins were investigated. The positions of the first excited electronic energy levels of these compounds were verified. AT-complex in the DNA, adenine groups in the RNA/oligoadenylate, and tryptophane groups in the proteins were examined as the main triplet electronic excitations traps. The changes in fluorescence and phosphorescence spectra of the co-systems mentioned above under variation of mutual concentrations of the oligoadenylate and protein macromolecules were observed. The spectral response of the binding of the oligoadenylate to the proteins (Human Albumin, Interferon, Immunoglobulin, Insulin) was fixed.

Key words: biomacromolecules, oligoadenylate, fluorescence, phosphorescence, electronic excitations energy transfer.

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